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Comparison of laboratory-developed and commercial monoclonal antibody-based sandwich enzyme-linked immunosorbent assays for almond (Prunusdulcis) detection and quantification.

Liu, C., G.S. Chhabra, J. Zhao,V.D. Zaffran, S.Gupta, K.H. Roux, T.M. Gradziel, S.K. Sathe, 2017. Comparison of laboratory-developed and commercial monoclonal antibody-based sandwich enzyme-linked immunosorbent assays for almond (Prunusdulcis) detection and quantification. J Food Sci. 82(10):2504-2515. A commercially available monoclonal antibody (mAb)-based direct sandwich enzyme-linked immunosorbent assay (ELISA) kit (BioFront Technologies, Tallahassee, Fla., U.S.A.) was compared with an in-house developed mAb 4C10-based ELISA for almond detection. The assays were comparable in sensitivity (limit of

2018-11-14T21:59:57+00:00Tags: , , |

Effects of the maillard reaction on the immunoreactivity of amandin in food matrices.

Chhabra, G.S., C. Liu, M. Su, M. Venkatachalam, K.H. Roux, S.K. Sathe, 2017. Effects of the maillard reaction on the immunoreactivity of amandin in food matrices. J Food Sci. 82(10):2495-2503. Amandin is the major storage protein and allergen in almond seeds. Foods, containing almonds, subjected to thermal processing typically experience Maillard browning reaction. The resulting destruction of amino groups, protein glycation, and/or denaturation may alter amandin immunoreactivity. Amandin immunoreactivity of variously processed almond containing

The effects of daily intake timing of almond on the body composition and blood lipid profile of healthy adults.

Liu, Y., H.-J. Hwang, H. Ryu, Y.-S. Lee, H.-S. Kim, H. Park, 2017. The effects of daily intake timing of almond on the body composition and blood lipid profile of healthy adults. Nutrition Research and Practice. 11(6):479-486. BACKGROUND/OBJECTIVES: Timing of almond intake during a day may result differently in the perspectives of body composition and changes of lipid profile. The current study was conducted to compare the effects of daily almond intake as a

Almond consumption and processing affects the composition of the gastrointestinal microbiota of healthy adult men and women: a randomized controlled trial.

Holscher, H.D., A.M. Taylor, K.S. Swanson, J.A. Novotny, D.J. Baer, 2018. Almond consumption and processing affects the composition of the gastrointestinal microbiota of healthy adult men and women: a randomized controlled trial. Nutrients 10(2), 126; https://doi.org/10.3390/nu10020126 Background: Almond processing has been shown to differentially impact metabolizable energy; however, the effect of food form on the gastrointestinal microbiota is under-investigated. Objective: We aimed to assess the interrelationship of almond consumption and processing on the gastrointestinal

Time and intervention effects of daily almond intake on the changes of lipid profile and body composition among free-living healthy adults.

Liu, X., H.-J. Hwang, H.-S. Kim, H. Park, 2018. Time and intervention effects of daily almond intake on the changes of lipid profile and body composition among free-living healthy adults. J Med Food. 21(4):340-347. Favorable health benefits of almond have been shown in several previous studies. However, repeated measures, randomized, controlled trials to investigate the changes due to almond intake based on the time effects have not yet been reported. The current study was

2018-11-14T21:34:52+00:00Tags: , , , |

Understanding the effect of particle size and processing on almond lipid bioaccessibility through microstructural analysis: from mastication to faecal collection.

Mandalari, G., M.L. Parker, M.M.-L. Grundy, T. Grassby, A. Smeriglio, C. Bisignano, R. Raciti, D. Trombetta, D.J. Baer, P.J. Wilde, 2018. Understanding the effect of particle size and processing on almond lipid bioaccessibility through microstructural analysis: from mastication to faecal collection.  Nutrients. 10, 213; doi:10.3390/nu10020213 We have previously reported on the low lipid bioaccessibility from almond seeds during digestion in the upper gastrointestinal tract (GIT). In the present study, we quantified the lipid released

Immunoreactivity of biochemically purified amandin from thermally processed almonds (PrunusdulcisL.).

Zaffran, V.D., S.K. Sathe, 2018. Immunoreactivity of biochemically purified amandin from thermally processed almonds (PrunusdulcisL.). J Food Sci. 83(7):1805-1809. Almond seeds were subjected to select thermal processing and amandin was purified from processed and unprocessed (control) seeds using cryoprecipitation. Amandin immunoreactivity was assessed using two murine monoclonal antibodies (mAbs)–4C10 and 4F10 detecting human IgE-relevant conformational and linear epitopes, respectively. Overall amandin immunoreactivity following thermal treatment ranged from 64.9% to 277.8% (4C10) and 81.3% to

2018-11-14T21:26:25+00:00Tags: , , |

Food allergen epitope mapping.

Liu, C., S.K. Sathe, 2018. Food allergen epitope mapping. J. Agric. Food Chem. 66:7238−7248. With the increased global awareness and rise in food allergies, a multifold interest in food allergens is evident. The presence of undeclared food allergens results in expensive food recalls and increased risks of anaphylaxis for the sensitive individuals. Regardless of the allergenic food, the immunogen needs to be identified and detected before making any efforts to inactivate/eliminate it. In type

2018-11-14T21:23:15+00:00Tags: , , |

Almonds and cardiovascular health: A review.

Kalita S., S. Khandelwal, J. Madan, H. Pandya, B. Sesikeran, K. Krishnaswamy, 2018. Almonds and cardiovascular health: A review. Nutrients. 10, 468; doi:10.3390/nu10040468 Several preventive strategies to reduce dyslipidemia have been suggested, of which dietary modification features as an important one. Dyslipidemia is a major risk factor for coronary heart disease and strategies to manage dyslipidemia have been shown to reduce the incidence of cardiovascular disease (CVD). Although there are proven pharmacological therapies to

2018-11-14T21:16:16+00:00Tags: , , |

Inositol phosphates: health implications, methods of analysis, and occurrence in plant foods.

Duong, Q.H., K.G. Lapsley, R.B. Pegg, 2018. Inositol phosphates: health implications, methods of analysis, and occurrence in plant foods. J. Food Bioact. 1:41-55. Inositol phosphates (InsPs), especially myo-inositol hexakisphosphate (InsP6), are important binders of phosphorus and minerals in plant seeds. However, they have long been considered as anti-nutritional components of plant foods due to their possible negative effects on the absorption of minerals and proteins in mammals. On the other hand, recent findings have

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