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Comparison of laboratory-developed and commercial monoclonal antibody-based sandwich enzyme-linked immunosorbent assays for almond (Prunusdulcis) detection and quantification.

Liu, C., G.S. Chhabra, J. Zhao,V.D. Zaffran, S.Gupta, K.H. Roux, T.M. Gradziel, S.K. Sathe, 2017. Comparison of laboratory-developed and commercial monoclonal antibody-based sandwich enzyme-linked immunosorbent assays for almond (Prunusdulcis) detection and quantification. J Food Sci. 82(10):2504-2515. A commercially available monoclonal antibody (mAb)-based direct sandwich enzyme-linked immunosorbent assay (ELISA) kit (BioFront Technologies, Tallahassee, Fla., U.S.A.) was compared with an in-house developed mAb 4C10-based ELISA for almond detection. The assays were comparable in sensitivity (limit of

2018-11-14T21:59:57+00:00Tags: , , |

Immunoreactivity of biochemically purified amandin from thermally processed almonds (PrunusdulcisL.).

Zaffran, V.D., S.K. Sathe, 2018. Immunoreactivity of biochemically purified amandin from thermally processed almonds (PrunusdulcisL.). J Food Sci. 83(7):1805-1809. Almond seeds were subjected to select thermal processing and amandin was purified from processed and unprocessed (control) seeds using cryoprecipitation. Amandin immunoreactivity was assessed using two murine monoclonal antibodies (mAbs)–4C10 and 4F10 detecting human IgE-relevant conformational and linear epitopes, respectively. Overall amandin immunoreactivity following thermal treatment ranged from 64.9% to 277.8% (4C10) and 81.3% to

2018-11-14T21:26:25+00:00Tags: , , |

Food allergen epitope mapping.

Liu, C., S.K. Sathe, 2018. Food allergen epitope mapping. J. Agric. Food Chem. 66:7238−7248. With the increased global awareness and rise in food allergies, a multifold interest in food allergens is evident. The presence of undeclared food allergens results in expensive food recalls and increased risks of anaphylaxis for the sensitive individuals. Regardless of the allergenic food, the immunogen needs to be identified and detected before making any efforts to inactivate/eliminate it. In type

2018-11-14T21:23:15+00:00Tags: , , |

Almond Allergy: An Overview on Prevalence, Thresholds, Regulations and Allergen Detection.

Mandalari, G., A.R. Mackie, 2018. Almond Allergy: An Overview on Prevalence, Thresholds, Regulations and Allergen Detection. Nutrients. 10(11), 1706; https://doi.org/10.3390/nu10111706 Food allergy has been on the increase for many years. The prevalence of allergy to different foods varies widely depending on type of food, frequency of consumption and geographic location. Data from the literature suggests that the prevalence of tree nut allergy is of the order of 1% in the general population. Almond is

2018-11-13T21:49:59+00:00Tags: , , |

Peanut and tree nut consumption during pregnancy and allergic disease in children—should mothers decrease their intake? Longitudinal evidence from the Danish National Birth Cohort.

Maslova, E., C. Granstrőm, S. Hansen, S.B. Petersen, M. Strøm, W.C. Willett, S.F. Olsen, 2012. Peanut and tree nut consumption during pregnancy and allergic disease in children—should mothers decrease their intake? Longitudinal evidence from the Danish National Birth Cohort. J Allergy Clin Immunol. 130:724-32.

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